Abstract Introduction: type 2 diabetes (T2DM) is a complex disease affected by lifestyle and genetic factors. Although the drugs currently used to treat T2DM have certain curative effects, they still have some adverse side effects. Therefore, it is urgent to find new effective drugs with few side effects to cure T2DM. Objective: to study the role of Inonotus obliquus (IO) in diabetic model mice. Methods: we used high-fat diet (HFD) combined with streptozocin (STZ) to establish a diabetic mouse model. Mice were divided into non-high-fat diet group (ND), diabetes model group (HFD + STZ) and IO-treated diabetes model group (IO). The mice in the IO group were orally treated with IO (150 mg/kg) at 10 ml/kg for five weeks. Body weight, glucose level, food intake and water consumption, glucose tolerance and insulin tolerance were evaluated in all mice. The pathological sections of liver, kidney and pancreas were observed by hematoxylin-eosin staining. Results: after IO administration, the blood glucose level, water consumption, low-density lipoprotein (LDL) and triacylglycerol (TG) levels of mice decreased. Compared with the HFD + STZ group, the number of normal islet iiiiiiii cells increased and focal necrosis of the liver was significantly reduced in the IO administration group. Conclusions: IO reduced the levels of blood glucose, restored body weight, and enhanced insulin sensitivity along with insulin tolerance and glucose tolerance in diabetic mice. Additionally, IO also reversed HFD and STZ-induced organ injury.

Resumen Introducción: la diabetes mellitus tipo 2 (T2DM) es una enfermedad compleja influenciada por el estilo de vida y los factores genéticos. En la actualidad, aunque los medicamentos para la diabetes tipo 2 tienen cierto efecto curativo, todavía tienen algunos efectos secundarios. Por lo tanto, es urgente encontrar nuevos medicamentos para la diabetes tipo 2 que tengan un buen efecto curativo y menos efectos secundarios. Objetivo: estudiar el papel del Inonotus obliquus (IO) en ratones diabéticos. Métodos: se estableció un modelo de ratón diabético con dieta de alto contenido en grasas (HFD) y estreptozocina (STZ). Los ratones se dividieron en el grupo de dieta no alta en grasas (ND), el grupo modelo de diabetes mellitus (HFD + STZ) y el grupo modelo de diabetes mellitus tratado con IO. Los ratones del grupo IO recibieron 10 ml/kg de IO (150 mg/kg) durante cinco semanas. Se observaron el peso corporal, el nivel de azúcar en sangre, la ingesta de alimentos, la ingesta de agua potable, la tolerancia a la glucosa y la tolerancia a la insulina de los ratones de cada grupo, y se estudiaron muestras de biopsias hepáticas, renales y pancreáticas mediante tinción de hematoxilina eosina. Resultados: los niveles de glucosa en sangre, el consumo de agua, la lipoproteína de baja densidad (LDL) y los triglicéridos (TG) disminuyeron después de la administración de IO. En comparación con el grupo HFD+STZ, el número de células β pancreáticas normales y la necrosis focal hepática disminuyeron significativamente en el grupo IO. Conclusiones: el IO redujo el nivel de glucosa en sangre, ayudó a recuperar el peso corporal y mejorar la sensibilidad a la insulina, la tolerancia a la insulina y la tolerancia a la glucosa en ratones diabéticos. Además, el IO revirtió el daño orgánico inducido por HFD y STZ.

Three new biphenyls were isolated from Garcinia multiflora. The structures of these biphenyls were elucidated by spectroscopic methods, and their rotavirus activity was evaluated.

Background Aspergillus niger has the ability to secrete feruloyl esterase. However, for economically viable industrial applications, it is necessary to increase their catalytic activities and/or protein yields to satisfy the increasing needs for feruloyl esterases. Results The gene AnFaeA that encodes a type A feruloyl esterase was successfully expressed in Pichia pastoris by a two-copy engineered yeast. After a screen in shaker flask, a one-copy strain GSKFA3 having the highest feruloyl esterase activity of 2.4 U/mL was obtained. Then, the pPICZaA-AnFaeA plasmid was transformed into GSKFA3 and the transformants were grown on YPDS plates with antibiotic Zeocin. After cultivation, a two-copy strain GSKZaFA20 with the highest feruloyl esterase activity of 15.49 U/mL was obtained. The expressed protein (recombinant AnFaeA) may be a glycoprotein with an apparent molecular weight of 40 kDa. It displayed the maximum activity at pH 6.0 and 50°C, and was stable at a pH range of 4.0-6.5 and at below 45°C. Its activity was not significantly affected by K+, Ca2 +, Mg2 +, Cu2 +, Zn2 +, Mn2 +, Na+ and EDTA, but activated by Fe2 +. The Km and Vmax toward 4-nitrophenyl ferulate were 5.5 mM and 69.0 U/mg, respectively. Conclusions The two-copy strain GSKZaFA20 showed a 4.4-fold increase in extracellular enzyme activity compared with the one-copy strain GSKFA3. Construction of two-copy strain improved secretion of recombinant AnFaeA in P. pastoris.

Three new chalcones were isolated from Desmodium oxyphyllum. Their structures were elucidated by spectroscopic methods including extensive 1D- and 2D-nuclear magnetic ressonance techniques. The compounds were evaluated for their cytotoxicity against five human tumor cell lines (NB4, A549, SHSY5Y, PC3, and MCF7). The results showed that the compounds exhibited weak cytotoxicity against some selected cell lines with IC50 values ranging from 3.6 to 8.9 μM.

New butyrolactones aspernolides C and D, along with two known butyrolactones (A and B) were isolated from the culture of the endophytic fungus Aspergillus versicolor. Their structures were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D nuclear magnetic ressonance techniques, and electronic circular dichroism. The butyrolactones aspernolides C and D were tested for their anti-tobacco mosaic virus activity. The results showed that butyrolactones aspernolides C and D exhibited moderate anti-tobacco mosaic virus activity with IC50 values of 64.2 and 88.6 μM, respectively.

The literature on development in economics and sociology has tended to focus on capital flows, investments, and, more recently, institutions as key causal factors. Nor does it consider important developmental synergies produced by the rising interactions between immigrant organizations and sending-country governments. Using data from a recently completed comparative study, we document these processes for two major countries of out-migration: Mexico and China. The study compiled inventories of migrant organizations from both countries in the United States, interviewed leaders of the major ones, and complemented these data with interviews with officials and community leaders in each sending country. Profiles of these transnational ties were constructed, exemplifying their increasing density and developmental impact at the local and national levels.

La literatura sobre el desarrollo en economía y sociología ha tendido a enfocarse en los flujos de capital, las inversiones y, más recientemente, en las instituciones como los principales factores causales. Tampoco considera las importantes sinergias para el desarrollo que producen las crecientes interacciones entre las organizaciones de inmigrantes y los gobiernos de los países de origen. Utilizando información de un estudio comparativo recientemente terminado, documentamos estos procesos para dos importantes países de emigración: México y China. El equipo del estudio generó inventarios de organizaciones de migrantes provenientes de los dos países hacia Estados, entrevistó a los líderes de las principales organizaciones y complementó estos datos con entrevistas a funcionarios y líderes comunitarios en cada uno de los países de origen.

This article provides an overview of contemporary trends in immigration to the United States and a descriptive analysis of gendered patterns of immigrants' economic incorporation. Since the 1970s, both legal and illegal female immigration to the United States has increased steadily, suggesting a trend toward more permanent settlement compared with that of the past. Today, more than half of the immigrants to the United States are female. Although these women often migrate for reasons similar to those of men (such as seeking better economic opportunities or escaping persecution and extreme hardships), their experiences with labor-market incorporation and family formation differ from those of men, raising new issues for the understanding of the role of gender in immigrant settlement and adaptation.

Este artículo presenta una visión general de las tendencias contemporáneas de inmigración a los Estados Unidos, y un análisis descriptivo por género de patrones de la incorporación económica de los inmigrantes. A partir de los setenta, la inmigración femenina legal e ilegal a los Estados Unidos ha aumentado ininterrumpidamente, sugiriendo una tendencia hacia un asentamiento más permanente, comparado con el del pasado. Hoy en día, más de la mitad de los inmigrantes a los Estados Unidos son mujeres. Aunque estas mujeres frecuentemente migran por razones similares a aquellas de los hombres (tales como la búsqueda de mejores oportunidades económicas o escapar de persecución o condiciones extremas), sus experiencias de incorporación al mercado de trabajo y formación familiar son diferentes a las de los hombres, generando nuevas preguntas para entender el papel del género en el asentamiento y adaptación de los inmigrantes.